AccuUrine DNA Preparation Kit
    AccuUrine DNA Preparation Kit
    The AccuUrine DNA Preparation Kit relies on the magnetic separation method to separate, concentrate, and purify DNA and generates DNA faster than centrifugation. Since no centrifugation is needed, the DNA can be extracted quickly and efficiently from the urine sample and the kit contains DNA binding beads with high binding capacity that separate, concentrate, and purify DNA to generate highly concentrated and high-quality DNA.

    AccuPrep

    AccuUrine DNA
    Preparation Kit

    Cat : ACN 06.50

    ·

    For the rapid preparation of high quality DNA from small amounts of non-precipitated fresh or frozen urine

    Input Amount

    750 ㎕

    Processing Time

    < 1 hour (incl. lysis time)

    Format

    Bead

    Elution Volume

    20 ~ 100 ㎕

    Yield

    ≥ 10 ng

    Convenience

    Magnetic bead-type format makes the process convenient

    Easy & Fast

    Quick and easy DNA extraction from urine sample without precipitation

    High Concentration & High Purity

    Highly concentrated and high-quality DNA extraction

    Safe

    No organic solvents make it safe to use

    Cost-effective

    Cost-effective and high quality compared to competitors

    Application

    PCR, Real Time PCR, NGS, Sequencing, Microarray, etc.

    Experimental Procedure

    01
    Urine,Saliva,CSF.. cell free body fluid
    02
    Take the supernatant only
    10
    Urine,Saliva,CSF.. cell free body fluid
    11
    Harvest the cells
    12
    Add Resuspension buffer
    03
    Take the supernatant only
    03
    Take the sample
    04
    Add Proteinase K & lysis buffer
    05
    Incubation at 60℃ for 1-2hr

    Pre-treatment steps(For Biofluidic DNA)

    01
    1.
    Urine, Saliva, CSF.. cell-free body fluid
    02
    2.
    Take the supernatant only
    03
    3.
    Take the sample
    04
    4.
    Add Proteinase K & Lysis buffer
    05
    5.
    Incubation at 56 ℃ for 15 mins

    Pre-treatment steps(For Cellular DNA)

    10
    1.
    Urine,S aliva, CSF.. cell free body fluid
    11
    2.
    Harvest the cells
    12
    3.
    Add Resuspension buffer
    04
    4.
    Take the supernatant only
    11
    5.
    Take the sample
    05
    6.
    Add Proteinase K & Lysis buffer
    05
    7.
    Incubation at 56 ℃ for 15 mins

    DNA Extraction steps

    01
    1.
    Add Binding buffer
    02
    2.
    Binding
    03
    3.
    Add Beads
    04
    4.
    Separation& Washing x2
    05
    5.
    Aspirate and dry
    06
    6.
    Elution
    07
    7.
    Transfer
    08
    8.
    Purified Urine DNA

    Experimental Data

    Exp_Prep_Urine_01_02
    Exp_Prep_Urine_03