AccuUrine DNA Preparation Kit
    AccuUrine DNA Preparation Kit

    AccuUrine DNA Preparation Kit는 소변 샘플에서 빠르고 간편하게 DNA 추출이 가능하고

    높은 Binding Capacity를 갖는 DNA Binding Bead 를 사용하는 자성 분리 방식으로
    DNA를 분리, 농축 및 정제하는 과정을 거치기 때문에 고농도, 고품질의 DNA를 추출할 수 있습니다.

    AccuPrep

    AccuUrine DNA
    Preparation Kit

    Cat : ACN 06.50

    ·

    For the rapid preparation of high quality DNA from small amounts of non-precipitated fresh or frozen urine

    Input Amount

    750 ㎕

    Processing Time

    < 1 hour (incl. lysis time)

    Format

    Bead

    Elution Volume

    20 ~ 100 ㎕

    Yield

    ≥ 10 ng

    Convenience

    Bead 방식으로 실험이 간편함

    Easy & Fast

    소변 샘플에서 침전 단계 없이 빠르고 쉽게 DNA 추출 가능

    High Concentration & High Purity

    고농도, 고품질의 소변 DNA 추출 가능

    Safe

    유기 용매를 사용하지 않아 안전함

    Cost-effective

    고가의 수입 키트보다 뛰어난 가성비와 품질

    Application

    PCR, Real Time PCR, NGS, Sequencing, Microarray, etc.

    Experimental Procedure

    01
    Urine,Saliva,CSF.. cell free body fluid
    02
    Take the supernatant only
    10
    Urine,Saliva,CSF.. cell free body fluid
    11
    Harvest the cells
    12
    Add Resuspension buffer
    03
    Take the supernatant only
    03
    Take the sample
    04
    Add Proteinase K & lysis buffer
    05
    Incubation at 60℃ for 1-2hr

    Pre-treatment steps(For Biofluidic DNA)

    01
    1.
    Urine, Saliva, CSF.. cell-free body fluid
    02
    2.
    Take the supernatant only
    03
    3.
    Take the sample
    04
    4.
    Add Proteinase K & Lysis buffer
    05
    5.
    Incubation at 56 ℃ for 15 mins

    Pre-treatment steps(For Cellular DNA)

    10
    1.
    Urine,S aliva, CSF.. cell free body fluid
    11
    2.
    Harvest the cells
    12
    3.
    Add Resuspension buffer
    04
    4.
    Take the supernatant only
    11
    5.
    Take the sample
    05
    6.
    Add Proteinase K & Lysis buffer
    05
    7.
    Incubation at 56 ℃ for 15 mins

    DNA Extraction steps

    01
    1.
    Add Binding buffer
    02
    2.
    Binding
    03
    3.
    Add Beads
    04
    4.
    Separation& Washing x2
    05
    5.
    Aspirate and dry
    06
    6.
    Elution
    07
    7.
    Transfer
    08
    8.
    Purified Urine DNA

    Experimental Data

    Exp_Prep_Urine_01_02
    Exp_Prep_Urine_03