AccuStool DNA Preparation Kit
AccuStool DNA Preparation Kit
The AccuStool DNA Preparation Kit relies on the magnetic separation method to separate, concentrate, and purify DNA and can be used for quick and easy DNA extraction compared to centrifugation. This kit contains ceramic beads that facilitate sample breakdown and generate highly concentrated and high-quality DNA.
AccuPrep
AccuStool DNA
Preparation Kit
Cat : ACN 13.50
·
For rapid and simple extraction of genomic DNA from mammalian stools
Input Amount
150 ~ 200 mg
Processing Time
< 1 hour (incl. lysis time)
Format
Bead
Elution Volume
20 ~ 100 ㎕
Yield
≤ 25 µg
Purity(A260/A280)
1.7 ~ 2.0
Convenience
Magnetic bead-type format makes the process convenient
High Concentration & High Purity
Highly concentrated and high-quality cfDNA extraction
Ceramic Bead
Ceramic beads included to increase DNA yield
Safe
No organic solvents make it safe to use
Cost-effective
Cost-effective and high quality compared to competitors
Application
PCR, Real Time PCR, NGS, Sequencing, Microarray, etc.
Experimental Procedure
Pre-treatment steps
1.
Stool
sampling
2.
Add Stool
ceramic bead
3.
Add Proteinase K
& Lysis buffer
4.
Mixing
5.
Incubation
at 65 ℃ for 30 mins
6.
Take the
supernatant only
DNA Extraction steps
1.
Add Binding
buffer
2.
Binding
3.
Add Beads
4.
Separation&
Washing x2
5.
Aspirate
and dry
6.
Elution
7.
Transfer
8.
Purified DNA
Experimental Data
