AccuDBS DNA Preparation Kit
    AccuDBS DNA Preparation Kit

    The AccuDBS DNA Preparation Kit is a spin-column type kit that enables the collection of highly pure and concentrated DNA from dried blood samples

    and is applicable with NGS that requires highly concentrated samples.

    AccuPrep

    AccuDBS DNA
    Preparation Kit

    Cat : ACN 40.50

    ·

    For the rapid preparation of high quality DNA from the DBS (Dry Blood Spot)

    Processing Time

    < 1 hour (incl. lysis time)

    Format

    Column

    Elution Volume

    < 50 ㎕

    Purity(A260/A280)

    1.7 ~ 2.0

    Convenience

    Quick and easy extraction within 1 hour

    High Concentration & High Purity

    Highly concentrated and high quality DNA extraction

    Easy

    Spin column type makes the kit easy to use for anyone

    Safe

    No organic solvents makes the kit safe to use

    Cost-effective

    Cost-effective and high-quality kits compared to similar high-cost imported products

    Application

    PCR, Real Time PCR, NGS, Sequencing, Microarray, etc.

    Experimental Procedure

    Pre-treatment steps

    추가_1
    1.
    Add Proteinase K
    & DLB buffer
    추가_2
    2.
    Take the sample
    추가_3
    3.
    Incubation at 60 ℃
    for 15 mins
    Pre treatment step 4_추가_4_허유경 사원 이미지_121x181
    4.
    Add BLB buffer
    추가_5
    5.
    Incubation at 60 ℃
    for 10 mins

    DNA Extraction steps

    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_1_Add Binding buffer
    1.
    Add EtOH
    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_2_DNA Binding
    2.
    DNA binding

    Spin

    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_3_Add Washing buffer 1
    3.
    Add Washing buffer ①

    Spin

    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_4_Add Washing buffer 2
    4.
    Add Washing buffer ②

    Spin

    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_5_Drying
    5.
    Drying

    Spin

    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_6_Add Elution buffer
    6.
    Add Elution buffer

    Spin

    수정 후_AccuBlood DNA Prep Kit_Experimental Procedure_DNA Extraction_7_Purified DNA
    7.
    Purified DNA

    Experimental Data

    Exp_Prep_DBS_01
    Exp_Prep_DBS_02_03