AccuBuccal DNA Preparation Kit
AccuBuccal DNA Preparation Kit
The AccuBuccal DNA Preparation Kit is a spin column-type kit that is the most universally known method for DNA extraction and purification. This kit can be used to quickly extract high-purity and high-yield DNA from oral epithelial cells.
AccuPrep
AccuBuccal DNA Preparation Kit
Cat : ACN 08.50
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For the rapid preparation of high quality DNA from human or other mammalian buccal (cheek) cells
Input Amount
200 ㎕
Processing Time
< 1 hour (incl. lysis time)
Format
Column
Elution Volume
20 ~ 100 ㎕
Yield
≥ 1 µg
Purity(A260/A280)
1.7 ~ 1.9
Convenience
Quick and easy extraction
High Concentration & High Purity
High-concentration and high-quality DNA extraction
Easy
Spin column-type format makes the kit user friendly
Safe
No organic solvents make it safe to use
Cost-effective
Cost-effective and high quality compared to competitors
Application
PCR, Real Time PCR, NGS, Sequencing, Microarray, etc.
Experimental Procedure
Pre-treatment steps
1.
Sampling
2.
Take the sample
3.
Add Proteinase K & Lysis buffer
4.
Incubation at 56 ℃ for 15 mins
DNA Extraction steps
1.
Add Binding buffer
2.
DNA Binding
Spin
3.
Add Washing buffer ①
Spin
4.
Add Washing buffer ②
Spin
5.
Drying
Spin
6.
Add Elution buffer
Spin
7.
Purified DNA
Experimental Data
