AccuCirculating DNA Preparation Kit
    AccuCirculating DNA Preparation Kit
    The AccuCirculating DNA Preparation Kit is a magnetic bead-type kit that can separate short segments of concentrated DNA from ≤ 500 ㎕ of plasma and 100 ~ 200 bp of serum. This kit can be used to quickly extract highly concentrated and high-quality cfDNA.

    AccuPrep

    AccuCirculating
    DNA Preparation Kit

    Cat : ACN 10.50

    ·

    For rapid and simple purification of cell-free circulating DNA from plasma/serum samples

    Sample Type

    Plasma, Serum

    Input Amount

    100 ~ 500 ㎕

    Processing Time

    < 1 hour

    Format

    Bead

    Elution Volume

    20 ~ 100 ㎕

    Yield

    ≥ 1 ng

    Convenience

    Magnetic bead-type format makes the process convenient

    Small Input Amount

    cfDNA extraction possible from small amounts of plasma and serum

    High Concentration & High Purity

    Highly concentrated and high-quality cfDNA extraction

    Patent Application

    First commercial product and patent in South Korea

    Application

    PCR, Real Time PCR, NGS, Sequencing, Microarray, etc.

    Experimental Procedure

    Pre-treatment steps

    01
    1.
    Whole blood

    Spin

    02
    2.
    Serum/Plasma Separation
    03
    3.
    Take the
    Serum/Plasma only
    04
    4.
    Take the sample
    05
    5.
    Add Proteinase K
    & Lysis buffer
    06
    6.
    Incubation at
    60 ℃ for 30 mins

    DNA Extraction steps

    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_1_Add Binding buf
    1.
    Add Binding buffer
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_2_Binding
    2.
    Binding
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_3_Add Beads
    3.
    Add Beads
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_4_Separation & Wa
    4.
    Separation & Washing x2
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_5_Aspirate & Dry
    5.
    Aspirate & Dry
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_6_Elution
    6.
    Elution
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_7_Transfer
    7.
    Transfer
    수정 후_AccuCirculating DNA Prep Kit_Experimental Procedure_DNA Extraction_8_Purified Cell Free DNA
    8.
    Purified Cell-free DNA

    Experimental Data

    Exp_Prep_Circulating_01_02