{"id":4297,"date":"2021-07-14T06:50:11","date_gmt":"2021-07-14T06:50:11","guid":{"rendered":"http:\/\/accugenelab.com\/accuffpe-dna-preparation-kit\/"},"modified":"2022-12-16T07:54:03","modified_gmt":"2022-12-16T07:54:03","slug":"accuffpe-dna-preparation-kit","status":"publish","type":"page","link":"https:\/\/accugenelab.com\/en\/products\/accuprep\/accuffpe-dna-preparation-kit\/","title":{"rendered":"AccuFFPE DNA Preparation Kit"},"content":{"rendered":"\t\t
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AccuFFPE DNA Preparation Kit<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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The AccuFFPE DNA Preparation Kit is a spin column-type kit that is the most universally known method for DNA extraction and purification.\nThe HiDeparaffinization Solution included in the kit minimizes DNA damage by removing the paraffin which leads to a relatively large amount of extracted DNA. This feature makes this kit ideal for amplification in the target region.\nThis kit can also be used to remove impurities (e.g. inhibitors, contaminants, cell debris, etc.) and extract highly concentrated and high-quality DNA.\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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AccuPrep<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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AccuFFPE DNA
Preparation Kit<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Cat : ACN 03.50<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u00b7<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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For the rapid preparation of high quality DNA from small amounts of FFPE tissues<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Input Amount<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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5 ~ 10 \u00b5m thickness<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Processing Time<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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< 2.5 hours (incl. lysis time)<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Format<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Column<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Elution Volume<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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20 ~ 100 \u3395<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Yield<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u2264 5 \u00b5g<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Purity(A260\/A280)<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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1.7 ~ 1.9<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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User-friendly features\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Small Input Amount<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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DNA extraction possible from small amounts of FFPE samples\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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High Concentration &
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Highly concentrated and high-quality cfDNA extraction\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Easy<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Spin column-type format makes the kit user friendly\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Safe<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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No organic solvents make it safe to use (HiDeparaffinization Solution)\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Patent Application<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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First commercial product and patent in South Korea\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Application<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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PCR, Real Time PCR, Sequencing,
Microarray, etc. <\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Experimental Procedure<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Pre-treatment steps<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Section or\nHomogenize<\/div><\/div><\/div>\n<\/div>\n
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Take sample(5 ~ 10 \u339b \nparaffin section) <\/div><\/div><\/div>\n<\/div>\n
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Add \nHideparaffinization Solution\n<\/div><\/div><\/div>\n<\/div>\n
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Add Proteinase K\n& Lysis buffer<\/div><\/div><\/div>\n<\/div>\n
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Spin<\/p>\nIncubation\nat 56 \u2103 for 1 - 2 hr<\/div><\/div><\/div>\n<\/div>\n

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Spin<\/p>\nIncubation \nat 90 \u2103 for 1 - 2 hr\n<\/div><\/div><\/div>\n<\/div>\n

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7.<\/div>
Take the blue\nlower phase solution\n<\/div><\/div><\/div>\n<\/div>\n<\/div>
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<\/image><\/g><\/g><\/svg><\/div><\/div>\n<\/div>\n<\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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DNA Extraction steps<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\"\uc218\uc815<\/a>
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Add Binding buffer\n<\/div><\/div><\/div>\n<\/div>\n
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Spin<\/p>\nDNA Binding<\/div><\/div><\/div>\n<\/div>\n

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Spin<\/p>\nAdd Washing buffer \u2460<\/div><\/div><\/div>\n<\/div>\n

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Spin<\/p>\nAdd Washing buffer \u2461\n<\/div><\/div><\/div>\n<\/div>\n

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Spin<\/p>\nDrying\n<\/div><\/div><\/div>\n<\/div>\n

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Spin<\/p>\nAdd Elution\nbuffer\n<\/div><\/div><\/div>\n<\/div>\n

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7.<\/div>
Purified DNA<\/div><\/div><\/div>\n<\/div>\n<\/div>
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Experimental Data<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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<\/image><\/g><\/g><\/svg><\/div><\/div>\n<\/div>\n<\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

AccuSaliva Collection kit AccuSaliva Collection kit The AccuFFPE DNA Preparation Kit is a spin column-type kit that is the most universally known method for DNA extraction and purification. The HiDeparaffinization Solution included in the kit minimizes DNA damage by removing the paraffin which leads to a relatively large amount of extracted DNA. This feature makes […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":4313,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/pages\/4297"}],"collection":[{"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/comments?post=4297"}],"version-history":[{"count":4,"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/pages\/4297\/revisions"}],"predecessor-version":[{"id":5975,"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/pages\/4297\/revisions\/5975"}],"up":[{"embeddable":true,"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/pages\/4313"}],"wp:attachment":[{"href":"https:\/\/accugenelab.com\/en\/wp-json\/wp\/v2\/media?parent=4297"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}